Nigute ushobora guhitamo umuco wimikorere ikoreshwa?
1. Menya uburyo bwo guhinga
Ukurikije uburyo butandukanye bwo gukura, selile zigabanyijemo ibyiciro bibiri: ingirabuzimafatizo zifatika hamwe ningirabuzimafatizo, kandi hariho ningirabuzimafatizo zishobora gukura haba mu kubahiriza no guhagarika, nka selile SF9.Ingirabuzimafatizo zitandukanye zifite ibisabwa bitandukanye kumico ikoreshwa.Utugingo ngengabuzima muri rusange dukoresha TC ikoreshwa na TC, mugihe ingirabuzimafatizo zidafite ibyo zisabwa, ariko ibikoreshwa na TC bivura nabyo bikwiranye no gukura kwa selile.Guhitamo ibikoreshwa bikwiye, uburyo bwumuco bwakagari bugomba kubanza kugenwa ukurikije ubwoko bwakagari.
2. Hitamo ubwoko bwibikoreshwa
Ibiryo bisanzwe bikoreshwa mumico ya selile birimo isahani yumuco wutugari, ibyokurya byumuco wutugari, flasks yumuco wumudugudu, icupa ryimodoka, inganda,Imiyoboro ya serologiya, nibindi. Ibi bikoresho bifite umwihariko wabyo mubijyanye numuco, uburyo bukoreshwa, nuburyo rusange.Icupa ryumuco numuco ufunze, ushobora kugabanya umwanda;icyapa cy'umuco kandipetrini umuco ufunguye igice, cyorohereza kugenzura no kugerageza buhoro buhoro, ariko nanone birashoboka cyane ko bitera kwandura bagiteri, bisaba abakora cyane.Ibikoreshwa bimwe nabyo bigomba gukoreshwa nibikoresho byihariye.Kurugero, shake ya selile ikeneye gukoresha vibrasiya ya shake kugirango selile zirusheho guhura nikirere, kandi uruganda rugizwe na 40 rukeneye ibikoresho byikora.Muri make, mugihe uhitamo ubwoko bwibikoreshwa, bigomba gusuzumwa neza hamwe nibikenewe mubigeragezo hamwe nibyifuzo byawe bwite.
1.Multi-nezaUmuco w'akagari.Usibye micro-plaque isanzwe yinjiza cyane, hashyizweho micro-plaque idasanzwe kugirango byorohereze umuco wa selile na organotypic.
1) Umubare wibyobo
Biterwa nurwego rwifuzwa, kandi hamwe nubufasha bwimashini.6, 12, 24 nibindi byapa bito byumuco utugari birashobora kongerwaho intoki.Kuri 96-nezaibyapa byumuco, nibyiza kugira ubufasha bwa pipeti y'amashanyarazi cyangwa imashini.
2) Imiterere yumwobo
Hasi y'iriba irashobora guhitamo kuba iringaniye (F-hepfo), kuzenguruka (U-hepfo), cyangwa gufatanwa, bitewe n'ubwoko bwakagari hamwe na porogaramu yo hasi.
3) Ibara ry'isahani
Ibara ryisahani isobekeranye nayo ifitanye isano rya hafi na porogaramu.Niba ingirabuzimafatizo zigaragaye hamwe na microscope itandukanye cyangwa nijisho ryonyine, icyapa kibonerana cyimeza cyiza kirashobora guhitamo.Nyamara, kubisabwa hanze yumucyo ugaragara (nka luminescence cyangwa fluorescence), amabara menshi-nezaibyapa byumuco(nk'umweru cyangwa umukara) birasabwa.
4) Kuvura hejuru
Ni ubuhe buryo bwo kuvura bwakagari kugirango uhitemo biterwa nuburyo urimo uhagarika ingirabuzimafatizo cyangwa ingirabuzimafatizo.
2.Umuco w'akagari: Agace k'umuco kari hagati ya cm 25-225, kandi muri rusange zahinduwe hejuru, zikwiranye no gufatira hamwe no gukura.225cm² na 175cm²umuco w'akagarizikoreshwa cyane mumico minini (nk'umuco w'akagari ka monoclonal, nibindi), 75cm² ikoreshwa cyane mubushakashatsi rusange (igice rusange, kubungabunga ingirabuzimafatizo, selile kubushakashatsi, nibindi), 25cm² ikoreshwa muburyo bushobora gukoreshwa kubyutsa selile cyangwa umuco mugihe hari selile nkeya, kandi mugihe ukora selile yibanze, amacupa menshi arashobora gukoreshwa kugirango wirinde kwanduzanya.
3.Erlenmeyer flask: Ugereranije nibikoreshwa nkinganda zingirabuzimafatizo hamwe nicupa rya roller selile, ifite agace gato k’umuco w'akagari kandi nigikoresho cyumuco wubukungu.Icupa ryumubiri wa flask rikozwe muri polyakarubone (PC) cyangwa ibikoresho bya PETG.Igishushanyo cyihariye cya mpandeshatu cyorohereza pipette cyangwa scraper selile kugera mu mfuruka y'icupa, bigatuma imikorere yumuco w'akagari yoroha.UwitekaErlenmeyer flaskingofero ikozwe mubikoresho bikomeye bya HDPE, bigabanijwemo kashe ya kashe hamwe numutwe uhumeka.Igifuniko cyo gufunga gikoreshwa mumico ifunze gaze na gaze.Igifuniko gihumeka gifite hydrophobic filter membrane hejuru yumucupa.Irinda kwinjira no gusohoka mikorobe, ikumira umwanda, kandi ikemeza ko hajyaho gaze, kugirango selile cyangwa bagiteri bikure neza.
Ingano isanzwe ya conical shakeflasks ya erlenmeyerni 125ml, 250ml, 500ml, 1000ml na3L, 5L ikora neza flasks ya erlenmeyer, Kugirango turebe ubushobozi bwikigereranyo no gusobanukirwa uko imikurire ikura, ingero zizacapishwa kumubiri w'icupa.Umuco w'akagari ugomba gukorwa mubidukikije.Kubwibyo rero, flask ya Erlenmeyer izavurwa bidasanzwe mbere yo gukoreshwa kugirango igere ku ngaruka za DNase, nta RNase, ndetse n’ibikomoka ku nyamaswa, bitanga uburyo bwiza bwo gukura kw ingirabuzimafatizo.ibidukikije.
4.Multi-layerUruganda rw'akagari: Uruganda rwakagari rukwiranye ninganda zinganda zinganda, nkinkingo, antibodiyite za monoclonal cyangwa inganda zimiti, ariko kandi kubikorwa bya laboratoire numuco munini w'akagari.Byoroshye kandi bifatika, irinde neza umwanda.Uruganda rwakagari rufite igifuniko gifunze: Igifuniko ntigifite umwobo uhumeka, kandi gikoreshwa cyane cyane mubihe bidafite dioxyde de carbone nka incubator na pariki.Uruganda ngengabuzima rufite igifuniko gifunze rushobora gukumira kwibasirwa na bagiteri zo hanze kandi bigafasha kurema imikurire myiza yo gukura kw ingirabuzimafatizo.Igifuniko gihumeka: Hariho umwobo uhumeka hejuru yumupfundikizo, bikoreshwa cyane mubidukikije bya karuboni.Imyobo ihumeka ituma karuboni ya dioxyde mu bidukikije yinjira mu ruganda, bigatuma habaho imikurire ikwiye yo gukura kw ingirabuzimafatizo.Hano hari igorofa 1, ibice 2, ibice 5, ibice 10, 40ingandairahari.
5.Gucuruza umucoicupa: Amacupa ya 2L & 5L Arakwiranye numuco utandukanye wimico yumudugudu hamwe numuco wa selile uhagarikwa, harimo selile Vero, selile HEK 293, selile CAR-T, MRC5, selile CEF, macrophage ya alcolar, selile myeloma, selile DF-1, ST selile, PK15 selile, Marc145 selile izindi selile zifatika.Irakwiriye kandi kumico ihamye yingirabuzimafatizo nka selile CHO, selile udukoko, selile BHK21 na selile MDCK.
3.Hitamo ibisobanuro byibikoreshwa.
Ubushakashatsi bunini bw'umuco w'akagari busaba ibikoreshwa hamwe n'umuco munini wo gushyigikirwa, mugihe ubushakashatsi buto-buke buhitamo ibikoreshwa hamwe n'akarere gato.Inganda zingirabuzimafatizo zikoreshwa cyane cyane mumico minini yingirabuzimafatizo, nkumusaruro winkingo, antibodiyite za monoclonal, inganda zimiti, nibindi.;amasahani yumuco, amasahani, hamwe na flasike birakwiriye kumico mito mito muri laboratoire;usibye umuco wo guhagarika selile, flask irashobora kandi Kubitegura hagati, kuvanga no kubika.Ukurikije igipimo cy'umuco w'akagari, menya neza ibintu byihariye bikoreshwa.
Ibikoresho bikoreshwa mu muco ukwiye ni byo byerekana ko imikurire ikura neza, kandi ni nurufunguzo rwo kwihutisha inzira yubushakashatsi no kwemeza ingaruka zumuco.Mu guhitamo, ibintu nkuburyo bwimico yumudugudu, igipimo cyumuco, hamwe na laboratoire bigomba gusuzumwa neza.dukeneye gukoresha ibindi bikoreshwa mugihe dukora umuco w'akagari, urugero,flake itwara CellDisk&umutwara wa serefegitire ya CellDisk,inama,kashe ya firime,imiyoboro, nibindi, Luoron arashobora kandi gutanga.
LuoRon Biotech Co., Ltd yibanda ku bushakashatsi, iterambere, kugurisha na serivisi zikoreshwa mu binyabuzima.Uruganda rutanga umusaruro rufite ubuso bwa metero kare 10,000.Ifite icyiciro cya 100.000 cyamahugurwa yumusaruro usukuye, icyiciro cya 10,000 cyamahugurwa yo guterana hamwe nubushakashatsi bwimbitse-bwuzuye.
Muri make, mugihe uhitamo ubwoko bwibikoreshwa, bigomba gusuzumwa neza hamwe nibikenewe mubigeragezo hamwe nibyifuzo byawe bwite.Nibyo, ni ngombwa kimwe guhitamo urubuga nka LuoRon rufite ibicuruzwa byujuje ubuziranenge kandi bitandukanye, ibicuruzwa bihamye, ubuziranenge na serivisi.LuoRon irashobora gutanga serivisi zuzuye zitanga amasoko imwe kubikoresho byubushakashatsi bwa laboratoire mubijyanye nubumenyi bwubuzima bwisi yose, inganda zimiti, kurengera ibidukikije, umutekano wibiribwa, ibigo bya leta, nubuvuzi bwamavuriro.
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