Fluorescence Umubare PCR Yerekana 96 ingero
Nkuguhitamo gukenewe kugirango isesengura ryinshi ryibinyabuzima bya molekuline, sisitemu nyayo ya PCR yakoreshejwe cyane mubice bitandukanye nkubushakashatsi bwa siyanse, gutahura ivuriro no gusuzuma, gupima ubuziranenge n’umutekano, hamwe n’ubushakashatsi.
Sisitemu nyayo-PCR
96
Ibiranga
• Imiyoboro igera kuri 6 ya fluorescence yemerera multiplex PCR.
• Kugabanya neza amabara menshi yambukiranya n'ingaruka, nta gukosora ROX bisabwa kugirango ugabanye icyitegererezo no gukoresha reagent
• Uburyo bushya bwo gusikana hamwe nuburyo bwo gukemura ibimenyetso byo gutandukanya ibimenyetso kugirango tunonosore ibyiyumvo
• Ikoranabuhanga ridasanzwe ryindishyi zubushyuhe kugirango ugabanye "inkurikizi"
• Porogaramu yorohereza abakoresha
• Ikoranabuhanga rishya rifite urumuri rurerure rwa LED rutanga ibisubizo byizewe
Ibipimo bya tekiniki
Sisitemu yo kugenzura ubushyuhe | |
Ubushobozi bw'icyitegererezo | 96 |
Ingano y'ibikorwa | 10-50 μl |
Ikoranabuhanga ryubushyuhe | Peltier |
Icyiza.Igipimo cyo gushyushya / ubukonje | 6.0 ° C / s |
Ubushyuhe buringaniye | 4 - 100 ° C. |
Ubushyuhe | ± 0.2 ° C. |
Ubushyuhe bumwe | ± 0.2 ℃ @ 60 ℃, ± 0.3 ℃ @ 95 ℃ |
Ubushyuhe buringaniye bwo gushiraho | 30–100 ° C. |
Ubushyuhe buhoro buhoro itandukaniro rishyiraho intera | 1 - 36 ° C. |
Sisitemu yo gutahura | |
Inkomoko yumucyo | 4/6 monochrome ikora neza LED |
Igikoresho cyo kumenya | PMT |
Uburyo bwo kumenya | Ikimenyetso cyakemuye igihe gitandukanya ikoranabuhanga |
Ibyishimo / gutahura intera ndende | 455-650nm / 510-715nm |
Imiyoboro ya Fluorescent | Imiyoboro ya 4/6 |
Irangi rishyigikiwe | FAM / SYBR Icyatsi, VIC / JOE / HEX / TET, ABY / NED / TAMRA / Cy3, KAMENA, ROX / Texas Umutuku, Umutuku wa Mustang, Cy5 / LIZ |
Ibyiyumvo | Kopi imwe |
Icyemezo | Inshuro 1.33 ikoporora umubare itandukaniro irashobora gutandukanywa muri plex imwe imwe |
Urwego rudasanzwe | Amabwiriza 10 yubunini bwa kopi |