Uwitekaerlenmeyer shake flaskni umuco udasanzwe wibikoresho byo guhagarika umuco, kandi birashobora no gukoreshwa mugutegura, kuvanga no kubika ibitangazamakuru bitandukanye.Uyu muco ukoreshwa ukoreshwa mugihe utera ingirabuzimafatizo za Jurkat.

Umurongo w'akagari ka Jurkat ukomoka ku maraso ya peripheri y'umuhungu w'imyaka 14 kandi ni selile yo guhagarika.Imirongo ngengabuzima ikomoka kuri Jurkat idafite genes zimwe ziraboneka muri banki z'umuco w'akagari.Imirongo idahwitse yumuntu T lymphocyte ikoreshwa cyane cyane mukwiga leukemia ikaze ya T, ibimenyetso bya selile T, hamwe na reseptor zitandukanye za chemokine zishobora kwandura virusi, cyane cyane imvugo ya sida.Ifite ibintu byinshi cyane mubushakashatsi bwibinyabuzima, nko gukoresha selile ya Jurkat kugirango yige M1-RNA ya ribonuclease P, hamwe nubushakashatsi bwa M1-RNA bwa anti-MHC icyiciro cya kabiri cyandika (CIITA) kugirango kibuze imvugo ya molekile ya MHC icyiciro cya kabiri hejuru yakagari.

Iyo utezimbere Jurkat selile muri erlenmeyer shake flasks, RPMI1640 iciriritse, 10% FBS irakenewe;ubushyuhe bugenzurwa kuri 37 ° C, 5% ya dioxyde de carbone, PH agaciro 7.2-7.4, umuco wa aseptic uhoraho.Ihanagura kandi wanduze hamwe na 75% alcool mbere yo kwimurira mu ntebe ya ultra-isukuye, ukuremo cryovial selile mu kigega cya azote yuzuye, uhite ubishyira mu bwogero bw’amazi bwa 37 ° C hanyuma uzunguze akayunguruzo ka selile kugirango uhite vuba.Hanyuma, nyuma ya centrifugation, kuvoma no kuvanga, nibindi, yashyizwe muri incubator ya selile kugirango ihingwe.

Ingirabuzimafatizo zumva neza ibidukikije.Iyo utera ingirabuzimafatizo za Jurkat muri selile ya shake ya erlenmeyer, isuku yumuntu igomba gukorwa neza, hagomba gukoreshwa reagile sterile, kandi hagomba gukurikizwa amahame yimikorere ya aseptic kugirango wirinde kwanduza bagiteri no kugira ingaruka kumikurire.