Uruganda rw'akagari ni igikoresho cy'umuco w'akagari, kigizwe nigikoresho cy’umuco w’akagari, gishobora kumenya ingano cyangwa ubwoko bw’umuco w’utugingo ngengabuzima, kandi gishobora gutahura neza neza ingirabuzimafatizo, zikwiranye n’imirima myinshi nk’uruganda rwa farumasi.hari uruganda 1 rw'utugari, uruganda 2 rw'utugari, ibice 5 & 10layers & 40layers zirahari.

1. Uruganda rumaze kwinjirira mumazi, umunwa wicupa ufata umunwa mugari, ushobora kuzuza vuba no gukusanya amazi, kandi ntibyoroshye kubyara umwuka mubi.Muri icyo gihe, igishushanyo kinini cyo mu kanwa gifasha cyane guhanahana gaze kandi kibereye umuco w’akagari.

2. Uruganda rusanzwe rufite ibikoresho bya 0.2m sterile ihumeka hamwe na capit yumwuka, bishobora gukoreshwa mumico itandukanye.Sterile vent caps ikoreshwa mubidukikije bya CO2, kandi imipira yumuyaga irashobora gukoreshwa muri incubator zisanzwe hamwe na parike yubusa ya CO2.Byongeye kandi, umupira wamazi urashobora guhitamo, bikaba byoroshye kugaburira amazi ya aseptic, kandi agacupa k'icupa gakwiranye no kugaburira amazi karashobora kandi gutegurwa ukurikije ibyo umukiriya asabwa.

3. Filime ihumeka yumutwe wamacupa yagenewe kuba hydrophobique, kandi ntabwo bizagira ingaruka kumyuka yumuyaga ningaruka zo guhumeka za firime ihumeka nyuma yo guhura namazi.

4. Uburyo bwo gutumiza mu mahanga butumizwa mu nganda z’akagari burashobora kwihanganira 1.5 PSI kugirango harebwe niba umuvuduko wa buri cyiciro cyibicuruzwa udatemba mugihe cyose cyakozwe.

Umuco w'akagari urakoreshwa no ku muco w'akagari gakurikiza umuco w'akagari.Filamentous kandi byihuse byinjira mugice cyo gukura kwa logarithmic. Mubisanzwe nyuma yiminsi mike, ubuso bwumuco burapfukiranwa kugirango habeho monolayeri yuzuye yingirabuzimafatizo, nka selile Vero, selile HEK 293, selile CAR-T, MRC5, selile CEF, macrophage ya alcolar. , selile myeloma, selile DF-1, selile ST, selile PK15, selile Marc145, nibindi byose byubahirijwe muburyo bwumuco.