Vacuolation ya selile bivuga isura ya vacuole (vesicles) yubunini butandukanye muri cytoplazme na nucleus ya selile yangiritse, kandi selile ni selile cyangwa reticular.Hariho impamvu nyinshi zitera iki kibazo.Turashobora kugabanya vacuolation ya selile muriumuco w'akagari flaskbike bishoboka binyuze mubikorwa bya buri munsi.
1. Emeza imiterere y'akagari: menya uko selile imeze mbere yo guhinga ingirabuzimafatizo, hanyuma ugerageze guhitamo ingirabuzimafatizo zifite umubare munini wibisekuru byo guhinga, kugirango wirinde icyuho bitewe no gusaza kwingirabuzimafatizo mugihe cyo guhinga.

2. Menya agaciro ka pH k'umuco uciriritse: wemeze neza pH yumuco wumuco na pH bisabwa ningirabuzimafatizo kugirango wirinde kugira ingaruka kumikurire ya selile kubera pH idakwiye.
3. Igenzura igihe cyo gusya cya trypsin: mugihe subculture, hitamo ubunini bukwiye bwa trypsin hanyuma uhitemo igihe gikwiye cyo gusya, kandi wirinde guhumeka cyane mugihe cyo kubaga.
4. Itegereze uko ingirabuzimafatizo imeze igihe icyo ari cyo cyose: Mugihe utera ingirabuzimafatizo, reba uko ingirabuzimafatizo ziri mu muco w’akagari igihe icyo ari cyo cyose kugira ngo umenye neza ko ingirabuzimafatizo zikenera intungamubiri zihagije kandi wirinde kwanduza ingirabuzimafatizo bitewe no kubura intungamubiri.
5. Gerageza gukoresha serumu yo mu nda ifite ubuziranenge bwiza kandi busanzwe, kuko serumu ikungahaye ku ntungamubiri kandi ifite ibintu bike bitera imbaraga, bishobora kwirinda ibibazo nkibi.
Ibikorwa byavuzwe haruguru birashobora kugabanya vacuolation yingirabuzimafatizo muri flask yumuco.Byongeye kandi, ibyangombwa bisabwa bigomba gushyirwa mubikorwa mugihe cyibikorwa kugirango bigabanye kwanduza ibintu bitandukanye.Niba ingirabuzimafatizo zigaragaye ko zanduye, zigomba kujugunywa mu gihe kugira ngo zitagira ingaruka ku bushakashatsi bwakurikiyeho.