Iyo selile tumaze umuco

urugandabaranduye, inyinshi murizo ziragoye kubyitwaramo.Niba selile zanduye zifite agaciro kandi ziragoye kongera kubona, uburyo bukurikira burashobora gukoreshwa mugukuraho.

1. Koresha antibiyotike

Antibiyotike ikora neza mukwica bagiteri muriinganda.Imiti yo guhuza ikora neza kuruta imiti yonyine.Imiti yo kwirinda irakorwa neza kuruta imiti nyuma yo kwanduza.Imiti yo kwirinda ikunze gukoresha antibiyotike ebyiri (penisiline 100u / mL wongeyeho streptomycine 100μg / mL).Nyuma yo kwanduza, uburyo bwo gukora isuku bugomba kuba bwikubye inshuro 5 kugeza 10 kurenza umubare usanzwe.Umuti ugomba gukoreshwa mumasaha 24 kugeza 48 nyuma yo kongerwaho, hanyuma ugasimbuzwa gahunda zisanzwe.Umuco utemba.Ubu buryo bushobora kuba ingirakamaro mugihe cyambere cyo kwanduza.Usibye penisiline na streptomycine, antibiyotike ikoreshwa irashobora kandi gushiramo gentamicin, kanamycin, polymyxin, tetracycline, nystatin, nibindi bikunze gukoreshwa ni 400 kugeza 800 μg / mL kanamycin cyangwa 200 μg / mL tetracycline.Ikigereranyo gihindurwa buri minsi 2 kugeza kuri 3 kandi kigahabwa ibisekuruza 1 kugeza 2 kugirango bivurwe.Mu myaka yashize, byavuzwe ko 4-fluoro, 2-hydroxyquinoline (Ciprofloxacin, Cip), inkomoko ya Pleu-romutilin (inkomoko ya Pleu-romutilin, BM-Cyclin2: BM-1 n’ibikomoka kuri tetracycline (BM-2)) Antibiyotike ni ingirakamaro mukwica mycoplasma iyo ikoreshejwe wenyine cyangwa hamwe.Izi antibiyotike eshatu zose zateguwe mubisubizo byibanda kuri 250X muri PBS kandi bibikwa kuri -20 ° C kugirango bikoreshwe nyuma.Imikoreshereze yibikoresho Cip ni 10 μg / mL, BM-1 ni 10 μg / mL, naho BM-2 ni 5μg / mL.Mugihe ukoresheje, banza wifuze umuco wanduye wanduye, ongeramo umuco wa RPMI1640 urimo BM-1, hanyuma wifuze umuco wumuco nyuma yiminsi 3, ongeramo umuco wa RPMI1640 urimo BM-2, numuco muminsi 4, nibindi muminsi 3 ikurikiranye .kuzenguruka, kugeza igihe bigaragajwe na 33258 fluorescent yerekana microscopi ko mycoplasma yakuweho, noneho umuco usanzwe wongewe kumuco no kunyuramo inshuro 3-4.

2. Kuvura ubushyuhe

Kwinjiza umuco wanduye wanduye kuri 41 ° C mumasaha 18 birashobora kwica mycoplasma, ariko bigira ingaruka mbi kuri selile.Kubwibyo, ikizamini kibanza kigomba gukorwa mbere yubuvuzi kugirango hamenyekane igihe cyo gushyuha gishobora kwica mycoplasma ku rugero runini kandi kigira ingaruka nke kuri selile.Ubu buryo rimwe na rimwe ntabwo bwizewe.Niba ubanje kuvurwa nibiyobyabwenge hanyuma ugashyuha kuri 41 ° C, ingaruka zizaba nziza.

3. Koresha serumu yihariye ya mycoplasma

Indwara ya Mycoplasma irashobora gukurwaho hamwe na 5% urukwavu mycoplasma immun serumu (hemagglutination titer 1: 320 cyangwa hejuru).Kuberako antibody yihariye ishobora kubuza imikurire ya mycoplasma, ihinduka nabi nyuma yiminsi 11 ivuwe na antiserum kandi igakomeza kuba mibi nyuma y amezi 5.ni bibi.Nyamara, ubu buryo burahangayikishije kandi ntabwo bworoshye kandi bwubukungu nko gukoresha antibiotike.

4. Ubundi buryo

Usibye uburyo bwavuzwe haruguru bwo gukuraho umwanda, hari nuburyo bwo gutera no kuboneza urubyaro mu nyamaswa, uburyo bwa macrophage phagocytose, uburyo bwo kongeramo bromouracil yanduye.amacupa yumucohanyuma ukabamurikira nurumuri, nuburyo bwo kuyungurura, nibindi, ariko byose nibibazo byinshi kandi ntacyo bikora.Kubwibyo, iyo mycoplasma yanduye imaze kubaho, keretse niba ifite agaciro gakomeye cyane, irajugunywa kandi ikongera ikagira umuco.

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