Iyo dukoresheje umuco wimikorere ya selile, duhora duhura nikibazo cyo gutambuka.Uyu munsi, tuzagusangiza muri make uburyo bwo gukoresha flasike yo hejuru-shake kugirango ucyure selile.Iyo dukoresheje amashanyarazi meza cyane. igice.
Uburyo bwo gutambuka hagati:
(1) Kwimura selile murishake flask hamwe numuco wo hagati kugeza kuri centrifuge tube ya centrifugation.
(2) Hagarika ndengakamere, ongeramo umuco mushya urwego kuri centrifuge tube naumuyoboroKuri Guhagarika Akagari.
(3) Kubara no gutera mumashanyarazi mashya.
Niba igice cyaremewe cyemewe, reka selile zahagaritswe gutura buhoro buhoro munsi ya flask yo hejuru cyane, fata 1/2/2/3 bya ndengakamere, hanyuma pipette kugirango ihagarike selile mbere yo kunyura.
Icyo dukeneye kwitondera mugihe cyo kubaga nuko trypsin igomba gushyuha mbere, kandi ubushyuhe bukaba bugera kuri 37 ° C.Umuvuduko wa centrifugation ugomba kuba ukwiye.Niba umuvuduko uri muke, selile ntishobora gutandukana neza.Niba umuvuduko wa centrifugation ari mwinshi cyane kandi igihe ni kirekire cyane, selile zizasunikwa, zitera kwangirika cyangwa no gupfa.Ingirabuzimafatizo zigomba kubahirizwa buri gihe, kandi niba habonetse umwanda, zigomba gukemurwa mugihe.
Igihe cyo kohereza: Mutarama-04-2023