Shake flask culturni murwego rwo kugenzura ibibazo n'umuco (ikizamini cyicyitegererezo), imiterere yumuco igomba kuba hafi ishoboka kumiterere yumusaruro wa fermentation, umutwaro wakazi ni munini, igihe kirekire, ibikorwa bigoye.Ibintu bigira ingaruka nziza muburyo bwo kunyeganyeza umuco wa flask cyane cyane ubushyuhe bwumuco, kunyeganyeza amplitude ya shaker, ingano yo kunyeganyeza flask, pH yumuco wo hagati, ubukonje bwikigereranyo, nibindi. Ubushyuhe bwumuco: ubushyuhe bwikura rya mycelium y'ibihumyo bitandukanye biribwa nabyo biratandukanye, ibyinshi mubushuhe bukwiye bwo gukura biri hagati ya 22 ℃ na 30 ℃, niba ubushyuhe bwumuco buri hasi cyane, imikurire ya mycelium iratinda;Iyo ubushyuhe bwari hejuru cyane, pellet ya mycelium yari irekuye kandi gake, kandi imbaraga nubuziranenge bwa pellet mycelium byagabanutse.

Kunyeganyeza inshuro nyinshi hamwe nuburyo bunoze bwo kunyeganyeza amacupa yipakurura: ibihumyo biribwa ni ibihumyo byindege, umuco wamazi, cyane cyane no kwinjiza ogisijeni yashonze mumico.Umwuka wa ogisijeni ushonga mu muco w’umuco wibasiwe ahanini nubwiza bwikigereranyo, ubwinshi bwamazi muri kontineri, inshuro zinyeganyega nibindi bintu.Inshuro yo kunyeganyega ni nini, flask yo kunyeganyega ni nto, ubunini bwikigereranyo bugera hejuru, umwuka wa ogisijeni ushonga wo hagati ni muremure, naho ubundi ukaba uri hasi.Mubisanzwe umuvuduko wikizunguruka ni 180-220 RPM / min, kwisubiraho ni 80-120 RPM / min, amplitude 6-7cm.

Ph yumuco iciriritse: PH yumuco iciriritse igira ingaruka itaziguye kwinjiza intungamubiri, ibikorwa bya enzyme no gukura kwa myelial pellet.PH yihariye igomba guhindurwa mbere yo guhagarika, ibihumyo byinshi biribwa muri pH 2.0-6.0.Mu rwego rwo gukumira impinduka zikomeye za PH mu muco w’umuco, karubone ya calcium, fosifate n’ibindi bintu bya buffer akenshi byongerwa mu muco.

Ubucucike buciriritse: Ubukonje buciriritse bugira ingaruka ku buryo butaziguye ingano ya ogisijeni yashonze muri yo, kandi ikagira ingaruka no ku miterere ya pelleti myelial.Ibisubizo byerekanaga ko iyo ubwiza bwumuco bwiyongereye, diameter ya pellet mycelium yagabanutse, umubare uriyongera, kandi umusaruro uriyongera.Kubwibyo, ukurikije ibisabwa byamazi yamazi kuri diameter ya pelleti ya mycelium, hagomba gushyirwaho uburyo bwumuco hamwe nubwiza runaka.Umuco w'akagari ni umurimo utoroshye, cyane cyane iyo ukeneye gutozwa hifashishijwe shitingi, nka shitingi ikora neza, igomba gutekereza cyane, kugirango habeho iterambere ry’umuco w'akagari.