Hano hari uduce twinshi twa selile nyuma ya lysis ya mashini yo guhagarika selile.Nigute ushobora gukuraho ibyo bice?Reka turebe uburyo butandukanye:

1. Koresha uburyo bwa dilution.Iyo ingirabuzimafatizo zimaze kuvangwa, zirashobora no kwiyongera, zizarushaho kuba nyinshi, kandi imyanda ya selile nayo igenda iba mike.
2. Hariho no gutura bisanzwe.Ingirabuzimafatizo zihuta vuba kuruta ibice byinshi :: Himura selile ihagarikwa muricentrifuge tube, kandi iyo selile nyinshi zirohamye, igisubizo cyo hejuru kirashobora gukurwaho, hanyuma kongerwaho igisubizo cyumuco kugirango uhagarike selile.Ubu buryo burashobora gukoreshwa inshuro nyinshi, mugihe buri gihe ushobora kureba niba bujuje ibyo usabwa munsi ya microscope.
3. Centrifuge yihutairashobora gukuraho imyanda, muri rusange 700g, 5mins
4. Iyo centrifuging, hashingiwe ko umubare w'utugingo ngengabuzima uhagije, gabanya igihe cya centrifugation, nka 5min, 1000rpm, aho kuba 3min, 1000rpm, hanyuma ukureho ndengakamere, kuko nérosose hamwe n'imyanda muri rusange biri mu ndengakamere!Iki gikorwa gikeneye gukorwa rimwe gusa mugihe cyo gukaraba mbere yubushakashatsi!

Nubwo hariho inzira nyinshi zo gukuraho imyanda ya selile, uzasanga centrifugation ari bumwe muburyo bwiza！

Kandi kubera ko ibimera ningirabuzimafatizo bifite urukuta rwa selile rugizwe na selile, hemicellulose na pectine, mubisanzwe birakenewe gusya umucanga wa quartz cyangwa ifu yikirahure hamwe nigisubizo kiboneye cyangwa kuvura hamwe na selile kugirango ugere kuntego.Gutandukanya ingirabuzimafatizo ya bagiteri biragoye cyane, kubera ko skeleton yose ya bagiteri ya selile skeleton mubyukuri ihuza isano ya peptidoglycan cystic macromolecules, irakomeye cyane.Uburyo busanzwe bwo kumena urukuta rwa bagiteri harimo gusya ultrasonic, gusya umucanga, gukuramo umuvuduko mwinshi cyangwa kuvura lysozyme.Nyuma ya tissue na selile zimenetse, buffer ikwiye iratoranijwe kugirango ikuremo poroteyine wifuza.Ibikoresho bidashonga nkibice bigize selile bivanwaho nacentrifugecyangwa kuyungurura.

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