Imiterere ya Bioreactor Microcarrier Yerekana Ishusho

Microcarrier ya Bioreactor

CellDeks1umutwarani mikorobe itwara mikorobe ikorwa no guhuza diethylaminochloroethane kuri micarike ya agarose, ituma ingirabuzimafatizo zoroha kuyihuza.Ni nacyo gikoresha mikorobe ikoreshwa cyane mumico yo guhagarika ingirabuzimafatizo., bikoreshwa cyane mubushakashatsi niterambere no kubyaza umusaruro ibinyabuzima.lt ibereye subculture ya selile Vero, selile HEK293, selile Mrc-5, selile CHO, selile BHK21, selile MDCK, selile ST, selile Marc145, selile SF9 / 21, nibindi lt nayo ikwiranye na selile CEF, selile PAM, myeloma selile n'umuco w'utugingo ngengabuzima nka CAR-T selile.

Abatwara bombi barashobora gukoreshwa mumico yingirabuzimafatizo.Ku nyungu zubukungu, guhora wakira virusi, nka virusi yibisazi, virusi yingurube, ukoresheje flake;Izindi virusi zikoresha sisitemu.Imikoreshereze yabatwara flake cyangwa abatwara sherfike igenwa ukurikije ibikoresho byabo nibikorwa

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Ibicuruzwa birambuye

Ibicuruzwa

Feature Ibiranga ibicuruzwa

Gutandukanya neza kandi byoroshye imico ningirabuzimafatizo, gusarura ibicuruzwa, parufe cyangwa kugaburira bikomeje.
.lt irashobora gutsimbatazwa mumashanyarazi manini manini ya bioreactors, ikoreshwa rimwe gusa hamwe na shake flasks kugirango itange ubuso buhagije bwo gukura kwakagari.
ltoroshye kwagura selile.Ingirabuzimafatizo zirashobora gusya ziva mubitwara hanyuma zigashyirwa mubitwara bishya, cyangwa umutwara mushya ashobora kongerwaho muburyo butaziguye kugirango bamenye uburyo "umupira-umupira" wo kwagura umuco.

Ibicuruzwa byingenzi

Kode ya kode	Microcarrier Celldex1
Kugaragara	imirongo yera, Impumuro nziza kandi itaryoshye
Ubucucike * (g / ml)	<1.045
Ingano y'ibice (pm)	Kuma: 50-100Muri saline: 145-240
Ubushobozi bwo guhanahana (mmol / g yumye)	1.4-1.6
Kubyimba * (ml / g uburemere bwumye)	17-22
Umuvuduko wo kugabanuka	10-12cm / min
Gutakaza kumisha	<10%
Ibirimo bya mikorobe (umubare wa koloni / g uburemere bwumye)	<100
Buri garama yuburemere bwumye irimo hafi ya microcarrière	4.3x106
Hafi.agace (amazi	4500cm2
Gusaba	Bikwiranye numuco wo guhagarika ingirabuzimafatizo zishingiye kuri anchorage, zikoreshwa mugukora inkingo na proteyine, nibindi.

Photomicrograph ya selile ya Vero ifite umuco muri CellDex1 kuri 24h

Ifoto ya selile Vero ifite umuco muri CellDex1 kuri 48h （320 x）

Ifoto ya selile Vero ifite umuco muri CellDex1 kuri 72h （320 x）

Para Ibipimo by'ibicuruzwa

Umubare wibicuruzwa	Ibisobanuro	Gupakiraimyaka	Icupa / urubanza
C100050	Ibiro by'akagari1	50 / icupa	40
C100250	Ibiro by'akagari1	250 / icupa	20
C100500	Ibiro by'akagari1	500 / icupa	10
C100001	Ibiro by'akagari1	1 kg/ icupa	4
C100005	Ibiro by'akagari1	5 kg/ icupa	1